Showing posts with label DNA laboratory. Show all posts
Showing posts with label DNA laboratory. Show all posts

Tuesday, 28 December 2010

DNA Sample Collection Procedure for DNA Testing

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Sample Collection Procedure

How to Perform a DNA Sample Collection for DNA Testing

DNA is the hereditary material or genetic instructions in humans and other organisms. Almost all cells in the body have the same DNA. DNA is located mainly in the cell nucleus but a small amount is located in the mitochondria. DNA extractions are from skin, muscle, bone marrow, saliva and cheek cells. DNA is used in solving crimes and for paternity testing.

Instructions

Step 1
Identify all items found inside your free kit for the collection of DNA for testing. Detailed instructions explaining the collection process along with 4 buccal swabs, 4 buccal swab envelopes to hold the samples and a business reply envelope for shipment the samples.
Step 2
Label the buccal swab envelope where indicated with the required information.
Step 3
Swab the inside of your cheek with the buccal swab provided in the kit. Use one of the swabs to rub against the inside of your cheek lining, rotate the swab while covering as much of the cheek area as possible.
Step 4
Put the used swab in pre-labeled envelope. Do not use the plastic wrapper that enclosed the swab; this can cause growing molds which will destroy the DNA sample.
Step 5
Repeat Step 3 and 4 with another swab for the same cheek. Following the same procedure use the remaining two swabs for the other cheek and seal the envelope with all 4 used swabs.
Step 6
Place the sealed envelope into the kit, put the entire collection kit in the business reply envelope provided and mail this package back to the laboratory for testing.
Step 7
Results from the paternity test should be available within 5-7 business days.

FAQ on DNA Sample collection prodedures:

The procedures for paternity testing, or DNA test, sample collection are quite simple. The following is a list of frequently asked questions that will help you find out about where to go, what to bring, who needs to show up, if there are age requirements, what types of samples there are, when to show up, and how your confidentiality is maintained.

Where are samples collected?
We will make every effort to ensure that you are scheduled at the most convenient site possible. We can even find a new location, if necessary, to meet your needs. To schedule an appointment for sample collections just call us or email us and a member of our Client Services staff will assist you.
We will provide you, or your client, with the location (usually a local laboratory or clinic), date, time for sample collection, and the name of a contact person at the site.

Are there any age requirements?
Paternity testing using DNA testing may be performed at any age. Age of the person(s) tested DOES NOT affect the accuracy of the paternity test / DNA test result in any way. We have successfully performed numerous tests involving newborn infants.

Who needs to provide a sample?
Usually, a sample is collected from the mother, child and alleged father. Under special circumstances, DNA testing / paternity testing may be performed using only samples from the child and alleged father. Mother sample can be optional.

What types of samples are collected?
In most cases DNA testing / paternity testing is done using cells from the inside of the mouth, which are collected with a cotton swab called a "buccal swab". The older more classic approach is to collect a very small sample of blood. The sample is small enough to allow even newborns to participate in testing. Newborns may also be tested using umbilical cord blood obtained at birth. Relationship testing can be performed on various discreet samples like Toothbrush, Hair sample, used bandage, contraceptives, nail clippings or any other samples where possibility of human dna exist. Please note that, our lab make best efforts to get good yield of DNA from these sample but some times it fails due to non sufficiency of DNA.

Are sample collections and tests confidential?
Yes. All information and all results are kept strictly confidential. Results are provided only to

Legal DNA Paternity Test in India

The type of paternity test you need will depend on what you intend to use the DNA test results for:
  • If you need paternity test results that can be used as a legal document (for example, to change the name on the birth certificate or to obtain child support and other benefits), a Legal DNA Test needs to be performed (described below).
  • However, if you need the test only for personal knowledge, a Home DNA Test willl suffice.
Unlike the Home DNA Test, where tested parties collect their own samples at their convenience, the Legal DNA Test follows a Chain of Custody documentation process to ensure that you receive accurate and legally defensible results. When you set up your case with us, we will coordinate a convenient sample collection appointment, during which a trained sample collector will complete all the necessary documentation to satisfy chain of custody requirements.

DNA Test Participants
In a standard DNA paternity test, the tested parties include a child, the alleged father, and the mother (called a trio).
The mother’s participation in the paternity test helps to exclude half of the child’s DNA, leaving the other half for comparison with the alleged father’s DNA. However, we can perform a paternity test without mother’s participation (called a motherless). A motherless test involves additional analysis, which we performs without any additional charge. Results are equally conclusive whether or not the mother participates. Motherless tests are guaranteed to have at least a 99.9% probability of paternity for inclusions and 100% for exclusion.

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Sample Collection :
We routinely use the painless buccal (cheek) swab to collect samples. We have the largest network of collection sites in the U.S., allowing our customers to choose a sample collection appointment most convenient for them. We also have extensive affiliations with laboratories worldwide for international sample collections.

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Peace of Minds
If you have determined that you do not need a DNA test for a legal matter — and only want to take a paternity test strictly for your own knowledge — a home DNA test might be an appropriate option for you. In a home DNA test, the tested parties collect the DNA samples themselves, at a time and place of their choosing. You can simply place an online paternity test order, and we will send you the kit in discreet packaging. Please note that the results of a home DNA test cannot be used as legal proof of paternity, because the identity of the persons tested cannot be verified
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Legal / Chain of custody :
As an AABB-accredited laboratory, we perform paternity tests and other DNA tests using a Chain of Custody documentation process to ensure results will be legally defensible, in case the results will be used in courts and other government agencies. This process proves and satisfies the following legal requirements:
  • Samples are collected by an impartial third party, such as a Doctor, clinic or laboratory.
  • The individuals tested are positively identified (i.e. They present a government-issued ID, and they are photographed and fingerprinted for records.).
  • The samples are carefully tracked and matched to each test participant throughout the entire DNA testing process.
The Chain of Custody process requires samples to be taken by a neutral third party. Thus, test participants would need to go to one of our sample collection facilities to be collected. The Chain of Custody process ensures that your results will be acceptable in court and other legal cases where paternity needs to be established.

DNA ANCESTRY TESTING

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ANCESTRY TESTING

If you would like to discover the history of your family dating back thousands of years, then OTS is able to offer you a range of DNA Ancestry Testing services. Through a simple DNA sample you will be able to trace the history of your ancestors and the area of the globe they originated from, as well as obtaining a record of their movements throughout the ages.
Why perform a DNA Ancestry Test?

There are various reasons to perform this test:
To increase your knowledge about the origins of your ancestors and their history dating back to thousands of years, for you and your children.

Need to know : for persons that have very limited or no information at all about their relatives, such as in the cases of adoption;

As a scientific tool : to confirm or clarify information obtained through genealogy research As a Gift : tracing their ancestry can be an unusual and fascinating gift to friends, relatives and loved ones.

Twin Zigosity Test

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Twin Zigosity Test

A Twin Zygosity Test is a DNA test that definitively shows whether twins are identical or fraternal.

During OB-GYN visits, the physician might be able to tell whether twins are identical or fraternal through ultrasound examination of the placenta. When the twins are born, pathological examination of the placenta can also be done to determine zgosity.

However, studies have shown that either method is not 100% accurate, and scientists recommend DNA testing to determine zygosity. There are also many cases in which medical records regarding zygosity have been lost, or doubt may have arisen because of the twins’ physical characteristics as the grow up.

In such cases, only a DNA test will be able to reveal the truth. A twin zygosity test compares the twins’ DNA profiles to see whether they match—an exact match proves that the twins are identical.

The results of a twin zygosity test may be used to satisfy personal curiosity as well as to help solve health problems for the twins later down the road. For example, in the event that a twin needs an organ or tissue transplant donor, the identical twin is a perfect choice

GRAND PARENTAGE TEST in Kolkata, Bengalore, Hyderabad, New Delhi

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GRAND PARENTAGE TEST

In situations where the alleged father is unavailable for testing such as he is deceased or unwilling to participate, the alleged father's parents can be tested to determine whether they are the true biological grandparents of the child. When conducting a grandparentage test, it is recommended to test both grandparents (the grandmother and the grandfather) in order to obtain the most conclusive results.
If this is not possible, a grandparentage test can be conducted by testing only one grandparent. The sample of a common parent (normally the Mother) is also recommended to strengthen the results of the test.